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验证数据展示

  • WB analysis using 68396-1-Ig

    WB analysis using 68396-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68396-1-Ig

    WB analysis using 68396-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68396-1-Ig

    IF Staining of HepG2 using 68396-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ALDOB antibody (68396-1-Ig, Clone: 3D2E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

ALDOB Monoclonal antibody

ALDOB Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68396-1-Ig

产品说明书

CloneNo. 3D2E4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat, rabbit

应用

WB, IF/ICC, ELISA

别名

ALDB, ALDOB, Liver type aldolase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat kidney tissue, human urine sample, rabbit liver tissue, rat liver tissue, mouse liver tissue, mouse kidney tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ALDOB Ready-To-Use IHC Kit. ALDOB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68396-1-Ig targets ALDOB in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat, rabbit
免疫原 ALDOB fusion protein Ag12823 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 aldolase B, fructose-bisphosphate
别名 ALDB, ALDOB, Liver type aldolase
计算分子量 316 aa, 35 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC029399
基因名称 ALDOB
Gene ID (NCBI) 229
RRIDAB_3085113
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05062
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA.

实验方案

Product Specific Protocols
WB protocol for ALDOB antibody 68396-1-IgDownload protocol
IF protocol for ALDOB antibody 68396-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols