CoraLite® Plus 488-conjugated ALDOB Monoclonal antibody
ALDOB Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, rabbit
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3D2E4
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ALDOB Ready-To-Use IHC Kit. ALDOB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68396-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68396 targets ALDOB in IF/ICC applications and shows reactivity with human, mouse, rat, rabbit samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat, rabbit |
| Immunogen | ALDOB fusion protein Ag12823 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | aldolase B, fructose-bisphosphate |
| Synonyms | Liver-type aldolase, Fructose-bisphosphate aldolase B, EC:4.1.2.13, ALDB, 3D2E4 |
| Calculated Molecular Weight | 316 aa, 35 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC029399 |
| Gene Symbol | ALDOB |
| Gene ID (NCBI) | 229 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05062 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ALDOB antibody CL488-68396 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |