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  • Featured Product
  • KD/KO Validated

ALKBH3 Polyclonal antibody, PBS Only

ALKBH3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12292-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 12292-1-AP (same clone as 12292-1-PBS)

    WB analysis using 12292-1-AP (same clone as 12292-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 12292-1-AP (same clone as 12292-1-PBS)

    IHC staining of human lung cancer using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of human prostate cancer using 12292-1-AP (same clone as 12292-1-PBS)

    IHC staining of human prostate cancer using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of human lung cancer using 12292-1-AP (same clone as 12292-1-PBS)

    IHC staining of human lung cancer using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of human pancreas cancer using 12292-1-AP (same clone as 12292-1-PBS)

    IHC staining of human pancreas cancer using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of rat stomach using 12292-1-AP (same clone as 12292-1-PBS)

    IHC staining of rat stomach using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH3 antibody included
  • IHC staining of mouse stomach using 12292-1-AP (same clone as 12292-1-PBS)

    IHC staining of mouse stomach using 12292-1-AP (same clone as 12292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH3 antibody included
Planning an IHC experiment? We recommend our IHCeasy ALKBH3 Ready-To-Use IHC Kit. ALKBH3 primary antibody included.

产品信息

12292-1-PBS targets ALKBH3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ALKBH3 fusion protein Ag2982 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alkB, alkylation repair homolog 3 (E. coli)
Synonyms
Calculated Molecular Weight 286 aa, 33 kDa
Observed Molecular Weight 22-25 kDa, 33kDa
GenBank Accession NumberBC015155
Gene Symbol ALKBH3
Gene ID (NCBI) 221120
RRIDAB_11125161
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96Q83
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.