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ALKBH5 Monoclonal antibody

ALKBH5 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67811-1-Ig

产品说明书

CloneNo. 2D2B2

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat and More (2)

应用

WB, IHC, ELISA

ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected in4T1 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ALKBH5 Ready-To-Use IHC Kit. ALKBH5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67811-1-Ig targets ALKBH5 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human, Mouse, Rat
文献引用反应性human, mouse
免疫原 ALKBH5 fusion protein Ag10490 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 alkB, alkylation repair homolog 5 (E. coli)
别名 ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1
计算分子量 458 aa, 51 kDa
观测分子量40-44 kDa
GenBank蛋白编号BC062339
基因名称 ALKBH5
Gene ID (NCBI) 54890
RRIDAB_2918574
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6P6C2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.

实验方案

Product Specific Protocols
WB protocol for ALKBH5 antibody 67811-1-IgDownload protocol
IHC protocol for ALKBH5 antibody 67811-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Apoptosis

NRP1 contributes to stemness and potentiates radioresistance via WTAP-mediated m6A methylation of Bcl-2 mRNA in breast cancer

Authors - Yang Wang
mouseWB

Cell Signal

FTO alleviates cerebral ischemia/reperfusion-induced neuroinflammation by decreasing cGAS mRNA stability in an m6A-dependent manner

Authors - Zhiyong Yu
humanIHC,WB

Sci Rep

ALKBH5 is a prognostic factor and promotes the angiogenesis of glioblastoma

Authors - Yugeng Fan
mouseWB

Acta Physiol (Oxf)

Landscape of transcriptome-wide m6A modification in diabetic liver reveals rewiring of PI3K-Akt signaling after physical exercise

Authors - Cong Chen
humanWB

Nat Commun

M6A reduction relieves FUS-associated ALS granules

Authors - Gaia Di Timoteo
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