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  • Featured Product
  • KD/KO Validated

ALKBH5 Monoclonal antibody, PBS Only

ALKBH5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D2B2

Cat no : 67811-1-PBS

Print datasheet

Synonyms

ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1

验证数据展示

  • WB analysis of HSC-T6 using 67811-1-Ig (same clone as 67811-1-PBS)

    WB analysis of HSC-T6 using 67811-1-Ig (same clone as 67811-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 67811-1-Ig (same clone as 67811-1-PBS)

    WB analysis of NIH/3T3 using 67811-1-Ig (same clone as 67811-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • WB analysis of 4T1 using 67811-1-Ig (same clone as 67811-1-PBS)

    WB analysis of 4T1 using 67811-1-Ig (same clone as 67811-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67811-1-Ig (same clone as 67811-1-PBS)

    WB analysis of HeLa using 67811-1-Ig (same clone as 67811-1-PBS)

    WB result of ALKBH5 antibody (67811-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALKBH5 transfected HeLa cells. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

    IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH5 antibody included
  • IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

    IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH5 antibody included
Planning an IHC experiment? We recommend our IHCeasy ALKBH5 Ready-To-Use IHC Kit. ALKBH5 primary antibody included.

产品信息

67811-1-PBS targets ALKBH5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ALKBH5 fusion protein Ag10490 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name alkB, alkylation repair homolog 5 (E. coli)
Synonyms ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1
Calculated Molecular Weight 458 aa, 51 kDa
Observed Molecular Weight40-44 kDa
GenBank Accession NumberBC062339
Gene Symbol ALKBH5
Gene ID (NCBI) 54890
RRIDAB_2918574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6P6C2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.