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  • Featured Product
  • KD/KO Validated

ALKBH5 Recombinant antibody, PBS Only

ALKBH5 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2K9

Cat No : 82083-1-PBS

Print datasheet

Synonyms

ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1

验证数据展示

  • WB analysis of HeLa using 82083-1-RR (same clone as 82083-1-PBS)

    WB analysis of HeLa using 82083-1-RR (same clone as 82083-1-PBS)

    WB result of ALKBH5 antibody (82083-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALKBH5 transfected HeLa cells. This data was developed using the same antibody clone with 82083-1-PBS in a different storage buffer formulation.

  • WB analysis using 82083-1-RR (same clone as 82083-1-PBS)

    WB analysis using 82083-1-RR (same clone as 82083-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82083-1-RR (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82083-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 82083-1-RR (same clone as 82083-1-PBS)

    IF Staining of U-251 using 82083-1-RR (same clone as 82083-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ALKBH5 antibody (82083-1-RR, Clone: 2K9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82083-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ALKBH5 Ready-To-Use IHC Kit. ALKBH5 primary antibody included.

产品信息

82083-1-PBS targets ALKBH5 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ALKBH5 fusion protein Ag10516 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name alkB, alkylation repair homolog 5 (E. coli)
Synonyms ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1
Calculated Molecular Weight 458 aa, 51 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC062339
Gene Symbol ALKBH5
Gene ID (NCBI) 54890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6P6C2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.