验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, A549 cells, K-562 cells, mouse brain tissue, rat brain tissue |
Positive IF/ICC detected in | U-251 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82083-1-RR targets ALKBH5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | ALKBH5 fusion protein Ag10516 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | alkB, alkylation repair homolog 5 (E. coli) |
别名 | RNA demethylase ALKBH5, EC:1.14.11.53, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, ALKBH 5, 2K9 |
计算分子量 | 458 aa, 51 kDa |
观测分子量 | 40-50 kDa |
GenBank蛋白编号 | BC062339 |
基因名称 | ALKBH5 |
Gene ID (NCBI) | 54890 |
RRID | AB_3086461 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q6P6C2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ALKBH5 antibody 82083-1-RR | Download protocol |
IF protocol for ALKBH5 antibody 82083-1-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |