• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ALPK1 Monoclonal antibody, PBS Only

ALPK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B8B5

Cat no : 67689-1-PBS

Print datasheet

Synonyms

Chromosome 4 kinase, ALPK, Alpha-protein kinase 1, Alpha protein kinase 1, alpha kinase 1

验证数据展示

  • WB analysis using 67689-1-Ig (same clone as 67689-1-PBS)

    WB analysis using 67689-1-Ig (same clone as 67689-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67689-1-Ig (ALPK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67689-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 67689-1-Ig (same clone as 67689-1-PBS)

    IHC staining of human heart using 67689-1-Ig (same clone as 67689-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67689-1-Ig (ALPK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67689-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 67689-1-Ig (same clone as 67689-1-PBS)

    IF Staining of THP-1 using 67689-1-Ig (same clone as 67689-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using ALPK1 antibody (67689-1-Ig, Clone: 2B8B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67689-1-PBS in a different storage buffer formulation.

产品信息

67689-1-PBS targets ALPK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ALPK1 fusion protein Ag5320 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alpha-kinase 1
Synonyms Chromosome 4 kinase, ALPK, Alpha-protein kinase 1, Alpha protein kinase 1, alpha kinase 1
Calculated Molecular Weight 139 kDa
Observed Molecular Weight139 kDa
GenBank Accession NumberBC060780
Gene Symbol ALPK1
Gene ID (NCBI) 80216
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96QP1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha-protein kinase 1, also known as alpha-kinase 1 (ALPK1), is associated with chronic kidney disease (CKD), myocardial infarction, gout and type 2 diabetes mellitus (DM). ALPK1 mutant has been reported to cause severe defects in motor co‐ordination in mice. ALPK1 enhances the expression of proinflammatory cytokines including IL-1β, IL-8 and TGF-β1 in cultured human THP1 and human embryonic kidney 293 (HEK293) cells (PMID: 31557402, PMID: 26275947). ALPK1 short isoform (108 kDa) was highly expressed in brain, spinal cord, heart, lung, spleen, thymus, small intestine, skin and testis, while detectable inskeletal muscle and kidney. ALPK1 large isoform (130 kDa) appeared in heart, lung, thymus and skin (PMID: 21208416).