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ALPL Polyclonal antibody, PBS Only

ALPL Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 11187-1-PBS

Print datasheet

Synonyms

Alkaline phosphatase, AKP2, AP, Alkaline phosphatase, tissue-nonspecific isozyme, Alkaline phosphatase tissue nonspecific isozyme

验证数据展示

  • WB analysis using 11187-1-AP (same clone as 11187-1-PBS)

    WB analysis using 11187-1-AP (same clone as 11187-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11187-1-AP (ALPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11187-1-AP (same clone as 11187-1-PBS)

    IHC staining of human colon cancer using 11187-1-AP (same clone as 11187-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11187-1-AP (ALPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALPL antibody included
  • FC experiment of HeLa using 11187-1-AP (same clone as 11187-1-PBS)

    FC experiment of HeLa using 11187-1-AP (same clone as 11187-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human ALPL (11187-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 11187-1-AP (same clone as 11187-1-PBS)

    IHC staining of mouse kidney using 11187-1-AP (same clone as 11187-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11187-1-AP (ALPL antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11187-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALPL antibody included
Planning an IHC experiment? We recommend our IHCeasy ALPL Ready-To-Use IHC Kit. ALPL primary antibody included.

产品信息

11187-1-PBS targets ALPL in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen ALPL fusion protein Ag1664 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alkaline phosphatase, liver/bone/kidney
Synonyms Alkaline phosphatase, AKP2, AP, Alkaline phosphatase, tissue-nonspecific isozyme, Alkaline phosphatase tissue nonspecific isozyme
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC021289
Gene Symbol ALPL
Gene ID (NCBI) 249
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05186
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).This antibody can bind the four mentioned alkaline phosphatases.