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ALPL Monoclonal antibody

ALPL Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E3C1

Cat No : 68719-1-Ig

Print datasheet

Synonyms

ALPL, AP TNAP, FLJ40094, FLJ93059, HOPS, TNAP, TNSALP

验证数据展示

  • WB analysis using 68719-1-Ig

    WB analysis using 68719-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68719-1-Ig (ALPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inpig kidney tissue, rabbit kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68719-1-Ig targets ALPL in WB, ELISA applications and shows reactivity with Human, pig, rabbit samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, pig, rabbit
Immunogen ALPL fusion protein Ag33678 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name alkaline phosphatase, liver/bone/kidney
Synonyms ALPL, AP TNAP, FLJ40094, FLJ93059, HOPS, TNAP, TNSALP
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC021289
Gene Symbol ALPL
Gene ID (NCBI) 249
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05186
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).

实验方案

Product Specific Protocols
WB protocol for ALPL antibody 68719-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols