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验证数据展示

  • WB analysis using 68719-1-Ig

    WB analysis using 68719-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68719-1-Ig (ALPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ALPL Monoclonal antibody

ALPL Monoclonal Antibody for WB, ELISA
Cat No. 68719-1-Ig

产品说明书

CloneNo. 2E3C1

宿主/亚型

Mouse / IgG2a

种属反应性

Human, pig, rabbit

应用

WB, ELISA

别名

ALPL, AP TNAP, FLJ40094, FLJ93059, HOPS, TNAP, TNSALP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig kidney tissue, rabbit kidney tissue
Planning an IHC experiment? We recommend our IHCeasy ALPL Ready-To-Use IHC Kit. ALPL primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68719-1-Ig targets ALPL in WB, ELISA applications and shows reactivity with Human, pig, rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, pig, rabbit
免疫原 ALPL fusion protein Ag33678 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 alkaline phosphatase, liver/bone/kidney
别名 ALPL, AP TNAP, FLJ40094, FLJ93059, HOPS, TNAP, TNSALP
计算分子量 57 kDa
观测分子量 80 kDa
GenBank蛋白编号BC021289
基因名称 ALPL
Gene ID (NCBI) 249
RRIDAB_3670412
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05186
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).

实验方案

Product Specific Protocols
WB protocol for ALPL antibody 68719-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols