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验证数据展示

  • WB analysis of human placenta using 84813-1-RR

    WB analysis of human placenta using 84813-1-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84813-1-RR (ALPPL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 84813-1-RR

    IHC staining of human placenta using 84813-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84813-1-RR (ALPPL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 84813-1-RR

    IHC staining of human placenta using 84813-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84813-1-RR (ALPPL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84813-1-RR

    Affinity and Kinetic Characterization of 84813-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84813-1-RR against Human ALPPL2 were performed. The affinity constant is below 1 pM.

ALPPL2 Recombinant antibody

ALPPL2 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84813-1-RR

产品说明书

CloneNo. 241867A12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ALPG, ALP-1, ALP1, Alkaline phosphatase, placental-like, Alkaline phosphatase, germ cell type

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84813-1-RR targets ALPPL2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 ALPPL2 fusion protein Ag13360 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alkaline phosphatase, placental-like 2
别名 ALPG, ALP-1, ALP1, Alkaline phosphatase, placental-like, Alkaline phosphatase, germ cell type
计算分子量 57 kDa
观测分子量70 kDa
GenBank蛋白编号BC014139
基因名称 ALPPL2
Gene ID (NCBI) 251
RRIDAB_3672137
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP10696
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALPPL2 (alkaline phosphatase placental-like 2), a member of the human alkaline phosphatase family, could be a potential biomarker for early diagnosis of Pancreatic ductal adenocarcinoma.

实验方案

Product Specific Protocols
WB protocol for ALPPL2 antibody 84813-1-RRDownload protocol
IHC protocol for ALPPL2 antibody 84813-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols