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验证数据展示

  • IF Staining of HEK-293 using CL488-24550

    IF Staining of HEK-293 using CL488-24550

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 AMOT antibody (CL488-24550) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AMOT Polyclonal antibody

AMOT Polyclonal Antibody for IF/ICC
Cat No. CL488-24550

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

KIAA1071, Angiomotin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 24550-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24550 targets AMOT in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen AMOT fusion protein Ag19784 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name angiomotin
Synonyms KIAA1071, Angiomotin
Calculated Molecular Weight 1084 aa, 118 kDa
Observed Molecular Weight 80 kDa, 130 kDa
GenBank Accession NumberBC130294
Gene Symbol AMOT
Gene ID (NCBI) 154796
RRIDAB_3672768
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4VCS5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Angiomotin belongs to the motin family of angiostatin binding protein. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells during the formation of new blood vessels. Most abundant expression was found in placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). The p130 isoform can interact with F-actin.This antibody recognizes both p130 and p80.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AMOT antibody CL488-24550Download protocol
Standard Protocols
Click here to view our Standard Protocols