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AMPD2 Monoclonal antibody, PBS Only

AMPD2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C4C1

Cat no : 67430-1-PBS

Print datasheet

Synonyms

AMP deaminase 2, AMP deaminase isoform L, AMPD2

验证数据展示

  • WB analysis using 67430-1-Ig (same clone as 67430-1-PBS)

    WB analysis using 67430-1-Ig (same clone as 67430-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

  • WB analysis using 67430-1-Ig (same clone as 67430-1-PBS)

    WB analysis using 67430-1-Ig (same clone as 67430-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67430-1-Ig (same clone as 67430-1-PBS)

    IF Staining of HepG2 using 67430-1-Ig (same clone as 67430-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMPD2 antibody (67430-1-Ig, Clone: 2C4C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67430-1-Ig (same clone as 67430-1-PBS)

    IHC staining of human liver cancer using 67430-1-Ig (same clone as 67430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67430-1-Ig (AMPD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67430-1-Ig (same clone as 67430-1-PBS)

    IHC staining of human liver cancer using 67430-1-Ig (same clone as 67430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67430-1-Ig (AMPD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

产品信息

67430-1-PBS targets AMPD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen AMPD2 fusion protein Ag8433 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name adenosine monophosphate deaminase 2 (isoform L)
Synonyms AMP deaminase 2, AMP deaminase isoform L, AMPD2
Calculated Molecular Weight879aa,101 kDa; 798aa,92 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC007711
Gene Symbol AMPD2
Gene ID (NCBI) 271
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01433
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Adenosine monophosphate deaminase (AMPD) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle. Three AMPDs are present in mammals named AMPD1/2/3. AMPD2 is the main activity present in adult human liver and is widely expressed in non-muscle tissues and cells (PMID: 8526848). AMPD2 has some isoforms with the molecular mass of 88-101 kDa.