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验证数据展示

  • WB analysis using 67430-1-Ig (same clone as 67430-1-PBS)

    WB analysis using 67430-1-Ig (same clone as 67430-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

  • WB analysis using 67430-1-Ig (same clone as 67430-1-PBS)

    WB analysis using 67430-1-Ig (same clone as 67430-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67430-1-Ig (same clone as 67430-1-PBS)

    IF Staining of HepG2 using 67430-1-Ig (same clone as 67430-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMPD2 antibody (67430-1-Ig, Clone: 2C4C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67430-1-Ig (same clone as 67430-1-PBS)

    IHC staining of human liver cancer using 67430-1-Ig (same clone as 67430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67430-1-Ig (AMPD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AMPD2 antibody included
  • IHC staining of human liver cancer using 67430-1-Ig (same clone as 67430-1-PBS)

    IHC staining of human liver cancer using 67430-1-Ig (same clone as 67430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67430-1-Ig (AMPD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67430-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AMPD2 antibody included

AMPD2 Monoclonal antibody, PBS Only

AMPD2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67430-1-PBS

产品说明书

CloneNo. 2C4C1

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AMP deaminase 2, AMP deaminase isoform L, AMPD2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy AMPD2 Ready-To-Use IHC Kit. AMPD2 primary antibody included.

产品信息

67430-1-PBS targets AMPD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 AMPD2 fusion protein Ag8433 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 adenosine monophosphate deaminase 2 (isoform L)
别名 AMP deaminase 2, AMP deaminase isoform L, AMPD2
计算分子量879aa,101 kDa; 798aa,92 kDa
观测分子量 101 kDa
GenBank蛋白编号BC007711
基因名称 AMPD2
Gene ID (NCBI) 271
RRIDAB_2882668
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ01433
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Adenosine monophosphate deaminase (AMPD) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle. Three AMPDs are present in mammals named AMPD1/2/3. AMPD2 is the main activity present in adult human liver and is widely expressed in non-muscle tissues and cells (PMID: 8526848). AMPD2 has some isoforms with the molecular mass of 88-101 kDa.