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验证数据展示

  • WB analysis using 67430-1-Ig

    WB analysis using 67430-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67430-1-Ig

    WB analysis using 67430-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67430-1-Ig

    IHC staining of human liver cancer using 67430-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67430-1-Ig (AMPD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AMPD2 antibody included
  • IHC staining of human liver cancer using 67430-1-Ig

    IHC staining of human liver cancer using 67430-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67430-1-Ig (AMPD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AMPD2 antibody included
  • IF Staining of HepG2 using 67430-1-Ig

    IF Staining of HepG2 using 67430-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMPD2 antibody (67430-1-Ig, Clone: 2C4C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

AMPD2 Monoclonal antibody

AMPD2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67430-1-Ig

产品说明书

CloneNo. 2C4C1

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, ELISA

别名

AMP deaminase 2, AMP deaminase isoform L, AMPD2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHSC-T6 cells, 4T1 cells, HeLa cells, HepG2 cells, L02 cells, Neuro-2a cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AMPD2 Ready-To-Use IHC Kit. AMPD2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:3000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67430-1-Ig targets AMPD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 AMPD2 fusion protein Ag8433 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 adenosine monophosphate deaminase 2 (isoform L)
别名 AMP deaminase 2, AMP deaminase isoform L, AMPD2
计算分子量879aa,101 kDa; 798aa,92 kDa
观测分子量 101 kDa
GenBank蛋白编号BC007711
基因名称 AMPD2
Gene ID (NCBI) 271
RRIDAB_2882668
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ01433
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adenosine monophosphate deaminase (AMPD) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle. Three AMPDs are present in mammals named AMPD1/2/3. AMPD2 is the main activity present in adult human liver and is widely expressed in non-muscle tissues and cells (PMID: 8526848). AMPD2 has some isoforms with the molecular mass of 88-101 kDa.

实验方案

Product Specific Protocols
WB protocol for AMPD2 antibody 67430-1-IgDownload protocol
IHC protocol for AMPD2 antibody 67430-1-IgDownload protocol
IF protocol for AMPD2 antibody 67430-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols