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验证数据展示

  • IF Staining of HepG2 using CL488-67430

    IF Staining of HepG2 using CL488-67430

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 AMPD2 antibody (CL488-67430, Clone: 2C4C1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated AMPD2 Monoclonal antibody

AMPD2 Monoclonal Antibody for IF/ICC
Cat No. CL488-67430

Print datasheet

CloneNo. 2C4C1

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Synonyms

AMP deaminase 2, AMP deaminase isoform L, AMPD2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AMPD2 Ready-To-Use IHC Kit. AMPD2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67430-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67430 targets AMPD2 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen AMPD2 fusion protein Ag8433 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name adenosine monophosphate deaminase 2 (isoform L)
Synonyms AMP deaminase 2, AMP deaminase isoform L, AMPD2
Calculated Molecular Weight879aa,101 kDa; 798aa,92 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC007711
Gene Symbol AMPD2
Gene ID (NCBI) 271
RRIDAB_2934543
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01433
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adenosine monophosphate deaminase (AMPD) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle. Three AMPDs are present in mammals named AMPD1/2/3. AMPD2 is the main activity present in adult human liver and is widely expressed in non-muscle tissues and cells (PMID: 8526848). AMPD2 has some isoforms with the molecular mass of 88-101 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AMPD2 antibody CL488-67430Download protocol
Standard Protocols
Click here to view our Standard Protocols