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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 12568-1-AP (same clone as 12568-1-PBS)

    WB analysis using 12568-1-AP (same clone as 12568-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP (same clone as 12568-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP (same clone as 12568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP (same clone as 12568-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP (same clone as 12568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12568-1-AP (same clone as 12568-1-PBS)

    IF Staining of HeLa using 12568-1-AP (same clone as 12568-1-PBS)

    Immunofluorescent analysis of HeLa cells using 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 12568-1-AP (same clone as 12568-1-PBS)

    IHC staining of human stomach using 12568-1-AP (same clone as 12568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

AMPK Gamma 2 Polyclonal antibody, PBS Only

AMPK Gamma 2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12568-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AMPK gamma2, PRKAG2, AMPK subunit gamma-2, AMPK subunit gamma 2, AAKG2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

12568-1-PBS targets AMPK Gamma 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AMPK Gamma 2 fusion protein Ag3214 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase, AMP-activated, gamma 2 non-catalytic subunit
别名 AMPK gamma2, PRKAG2, AMPK subunit gamma-2, AMPK subunit gamma 2, AAKG2
计算分子量 569 aa, 63 kDa
观测分子量 63-70 kDa, 38 kDa
GenBank蛋白编号BC020540
基因名称 PRKAG2
Gene ID (NCBI) 51422
RRIDAB_2252960
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UGJ0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma2 is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart.