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AMPK Gamma 2 Polyclonal antibody, PBS Only

AMPK Gamma 2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12568-1-PBS

Print datasheet

Synonyms

AMPK gamma2, PRKAG2, AMPK subunit gamma-2, AMPK subunit gamma 2, AAKG2

验证数据展示

  • WB analysis using 12568-1-AP (same clone as 12568-1-PBS)

    WB analysis using 12568-1-AP (same clone as 12568-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP (same clone as 12568-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP (same clone as 12568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP (same clone as 12568-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 12568-1-AP (same clone as 12568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12568-1-AP (same clone as 12568-1-PBS)

    IF Staining of HeLa using 12568-1-AP (same clone as 12568-1-PBS)

    Immunofluorescent analysis of HeLa cells using 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 12568-1-AP (same clone as 12568-1-PBS)

    IHC staining of human stomach using 12568-1-AP (same clone as 12568-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12568-1-PBS in a different storage buffer formulation.

产品信息

12568-1-PBS targets AMPK Gamma 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AMPK Gamma 2 fusion protein Ag3214 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Synonyms AMPK gamma2, PRKAG2, AMPK subunit gamma-2, AMPK subunit gamma 2, AAKG2
Calculated Molecular Weight 569 aa, 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC020540
Gene Symbol PRKAG2
Gene ID (NCBI) 51422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGJ0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma2 is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart.