验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse eye tissue, PC-3 cells, Neuro-2a cells, mouse heart tissues, rat heart tissues |
| Positive IHC detected in | human stomach tissue, human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
12568-1-AP targets AMPK Gamma 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | AMPK Gamma 2 fusion protein Ag3214 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase, AMP-activated, gamma 2 non-catalytic subunit |
| 别名 | AMPK gamma2, PRKAG2, AMPK subunit gamma-2, AMPK subunit gamma 2, AAKG2 |
| 计算分子量 | 569 aa, 63 kDa |
| 观测分子量 | 63-70 kDa, 38 kDa |
| GenBank蛋白编号 | BC020540 |
| 基因名称 | PRKAG2 |
| Gene ID (NCBI) | 51422 |
| RRID | AB_2252960 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UGJ0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma2 is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AMPK Gamma 2 antibody 12568-1-AP | Download protocol |
| IHC protocol for AMPK Gamma 2 antibody 12568-1-AP | Download protocol |
| IF protocol for AMPK Gamma 2 antibody 12568-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Med Chem Identification of Pyruvate Carboxylase as the Cellular Target of Natural Bibenzyls with Potent Anticancer Activity against Hepatocellular Carcinoma via Metabolic Reprogramming. | ||
J Cardiol Identification and functional analysis of a novel PRKAG2 mutation responsible for Chinese PRKAG2 cardiac syndrome reveal an important role of non-CBS domains in regulating the AMPK pathway. |
