- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated AMPK Alpha Monoclonal antibody
AMPK Alpha Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, Mouse and More (1)
Applications
IF-P and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1H8E1
验证数据展示
经过测试的应用
| Positive IF-P detected in | human breast cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy PRKAA1 Ready-To-Use IHC Kit. PRKAA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66536-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-66536 targets AMPK Alpha in IF-P applications and shows reactivity with Human, Rat, Mouse samples.
| Tested Applications | IF-P Application Description |
| Cited Applications | IF |
| Tested Reactivity | Human, Rat, Mouse |
| Cited Reactivity | mouse |
| Immunogen | AMPK Alpha fusion protein Ag24744 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | protein kinase, AMP-activated, alpha 1 catalytic subunit |
| Synonyms | AMPK, AMPK alpha, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1 |
| Calculated Molecular Weight | 63 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | BC012622 |
| Gene Symbol | AMPK Alpha 1 |
| Gene ID (NCBI) | 5562 |
| RRID | AB_2883352 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13131 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 AMPK Alpha antibody CL488-66536 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |