验证数据展示
经过测试的应用
| Positive IF-P detected in | human breast cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy PRKAA1 Ready-To-Use IHC Kit. PRKAA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66536-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-66536 targets AMPK Alpha in IF-P applications and shows reactivity with Human, Rat, Mouse samples.
| 经测试应用 | IF-P Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | Human, Rat, Mouse |
| 文献引用反应性 | mouse |
| 免疫原 | AMPK Alpha fusion protein Ag24744 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase, AMP-activated, alpha 1 catalytic subunit |
| 别名 | AMPK, AMPK alpha, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1 |
| 计算分子量 | 63 kDa |
| 观测分子量 | 64 kDa |
| GenBank蛋白编号 | BC012622 |
| 基因名称 | AMPK Alpha 1 |
| Gene ID (NCBI) | 5562 |
| RRID | AB_2883352 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q13131 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 AMPK Alpha antibody CL488-66536 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |