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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AMPK Alpha Monoclonal antibody

AMPK Alpha Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse and More (1)

Applications

IF-P and More (1)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H8E1

Cat no : CL488-66536

Print datasheet

Synonyms

AMPK, AMPK alpha, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1

验证数据展示

  • IF Staining of human breast cancer using CL488-66536

    IF Staining of human breast cancer using CL488-66536

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-66536 (AMPK alpha antibody) at dilution of 1:100.

  • IF Staining of human breast cancer using CL488-66536

    IF Staining of human breast cancer using CL488-66536

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-66536 (AMPK alpha antibody) at dilution of 1:100.

经过测试的应用

Positive IF-P detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy PRKAA1 Ready-To-Use IHC Kit. PRKAA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66536-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-66536 targets AMPK Alpha in IF-P applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications IF-P Application Description
Cited ApplicationsIF
Tested Reactivity Human, Rat, Mouse
Cited Reactivitymouse
Immunogen AMPK Alpha fusion protein Ag24744 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein kinase, AMP-activated, alpha 1 catalytic subunit
Synonyms AMPK, AMPK alpha, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC012622
Gene Symbol AMPK Alpha 1
Gene ID (NCBI) 5562
RRIDAB_2883352
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13131
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AMPK Alpha antibody CL488-66536Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Rep

Downregulation of hepatic ceruloplasmin ameliorates NAFLD via SCO1-AMPK-LKB1 complex

Authors - Liping Xie
View Article