验证数据展示
经过测试的应用
| Positive WB detected in | human saliva, tissue, pig pancreas tissue, rat pancreas tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:600-1:2400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66133-1-Ig targets Pancreatic alpha-amylase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, rat, pig |
| 文献引用反应性 | human, pig |
| 免疫原 | Pancreatic alpha-amylase fusion protein Ag8819 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | amylase, alpha 2A (pancreatic) |
| 别名 | AMY2A, Amylase Alpha, Amylase, alpha, AMY2B, AMY2 |
| 计算分子量 | 511 aa, 58 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC007060 |
| 基因名称 | Amylase Alpha |
| Gene ID (NCBI) | 279 |
| RRID | AB_2881532 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04746 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258). It has a hydrolase activity acting on glycosyl bonds. AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956).This antibody can recognize amylase alpha.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Pancreatic alpha-amylase antibody 66133-1-Ig | Download protocol |
| IHC protocol for Pancreatic alpha-amylase antibody 66133-1-Ig | Download protocol |
| IF protocol for Pancreatic alpha-amylase antibody 66133-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Physiol Gastrointest Liver Physiol Is the intestinal mucous layer a NAtural Deep Eutectic Solvent (NADES) based digestion matrix | ||
Biochem Biophys Rep Multiple cystic sphere formation from PK-8 cells in three-dimensional culture | ||
J Proteome Res Proteomic Analysis of Differences in the Freezability of Porcine Sperm Identifies α-Amylase As a Key Protein |
