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验证数据展示

  • WB analysis of human saliva using 66133-1-Ig (same clone as 66133-1-PBS)

    WB analysis of human saliva using 66133-1-Ig (same clone as 66133-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66133-1-Ig (same clone as 66133-1-PBS)

    IHC staining of human pancreas cancer using 66133-1-Ig (same clone as 66133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66133-1-Ig (Amylase, alpha antibody at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66133-1-Ig (same clone as 66133-1-PBS)

    IHC staining of human pancreas cancer using 66133-1-Ig (same clone as 66133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66133-1-Ig (Amylase, alpha antibody at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

  • IF Staining of BxPC-3 using 66133-1-Ig (same clone as 66133-1-PBS)

    IF Staining of BxPC-3 using 66133-1-Ig (same clone as 66133-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using Amylase Alpha antibody (66133-1-Ig, Clone: 2F1G8 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66133-1-PBS in a different storage buffer formulation.

Pancreatic alpha-amylase Monoclonal antibody, PBS Only

Pancreatic alpha-amylase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66133-1-PBS

产品说明书

CloneNo. 2F1G8

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AMY2A, Amylase Alpha, Amylase, alpha, AMY2B, AMY2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66133-1-PBS targets Pancreatic alpha-amylase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat, pig
免疫原 Pancreatic alpha-amylase fusion protein Ag8819 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 amylase, alpha 2A (pancreatic)
别名 AMY2A, Amylase Alpha, Amylase, alpha, AMY2B, AMY2
计算分子量 511 aa, 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC007060
基因名称 Amylase Alpha
Gene ID (NCBI) 279
RRIDAB_2881532
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04746
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258). It has a hydrolase activity acting on glycosyl bonds. AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956).This antibody can recognize amylase alpha.