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AMZ2 Polyclonal antibody

AMZ2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16664-1-AP

Print datasheet

Synonyms

EC:3.4.-.-, BM-014, Archeobacterial metalloproteinase-like protein 2, Archaemetzincin-2, Archaemetzincin 2

验证数据展示

  • IHC staining of human liver using 16664-1-AP

    IHC staining of human liver using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16664-1-AP (AMZ2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 16664-1-AP

    IF Staining of HepG2 using 16664-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 16664-1-AP

    FC experiment of HepG2 using 16664-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug AMZ2 Polyclonal antibody (16664-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HepG2 using 16664-1-AP

    WB analysis of HepG2 using 16664-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16664-1-AP (AMZ2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 16664-1-AP

    IHC staining of human testis using 16664-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inHepG2 cells, human brain tissue
Positive IHC detected inhuman liver tissue, human heart tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16664-1-AP targets AMZ2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AMZ2 fusion protein Ag10141 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name archaelysin family metallopeptidase 2
Synonyms EC:3.4.-.-, BM-014, Archeobacterial metalloproteinase-like protein 2, Archaemetzincin-2, Archaemetzincin 2
Calculated Molecular Weight 360 aa, 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC056271
Gene Symbol AMZ2
Gene ID (NCBI) 51321
RRIDAB_1850886
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86W34
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMZ2(archaelysin family metallopeptidase 2) is a zinc metalloprotease. Its calculated molecular weight and observed molecular weight are both 41kDa.

实验方案

Product Specific Protocols
WB protocol for AMZ2 antibody 16664-1-APDownload protocol
IHC protocol for AMZ2 antibody 16664-1-APDownload protocol
IF protocol for AMZ2 antibody 16664-1-APDownload protocol
FC protocol for AMZ2 antibody 16664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols