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验证数据展示

  • IF Staining of HepG2 using CL488-16664

    IF Staining of HepG2 using CL488-16664

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 AMZ2 antibody (CL488-16664) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HepG2 using CL488-16664

    FC experiment of HepG2 using CL488-16664

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated AMZ2 Polyclonal antibody (CL488-16664)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HepG2 using CL488-16664

    IF Staining of HepG2 using CL488-16664

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 AMZ2 antibody (CL488-16664) at dilution of 1:200.

CoraLite® Plus 488-conjugated AMZ2 Polyclonal antibody

AMZ2 Polyclonal Antibody for IF/ICC
Cat No. CL488-16664

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

EC:3.4.-.-, BM-014, Archeobacterial metalloproteinase-like protein 2, Archaemetzincin-2, Archaemetzincin 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 16664-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16664 targets AMZ2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 AMZ2 fusion protein Ag10141 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 archaelysin family metallopeptidase 2
别名 EC:3.4.-.-, BM-014, Archeobacterial metalloproteinase-like protein 2, Archaemetzincin-2, Archaemetzincin 2
计算分子量 360 aa, 41 kDa
观测分子量 41 kDa
GenBank蛋白编号BC056271
基因名称 AMZ2
Gene ID (NCBI) 51321
RRIDAB_3672658
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86W34
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMZ2 (archaelysin family metallopeptidase 2) is a zinc metalloprotease. Its calculated molecular weight and observed molecular weight are both 41kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AMZ2 antibody CL488-16664Download protocol
FC protocol for CL Plus 488 AMZ2 antibody CL488-16664Download protocol
Standard Protocols
Click here to view our Standard Protocols