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验证数据展示

  • IF Staining of NIH/3T3 using CL488-67577

    IF Staining of NIH/3T3 using CL488-67577

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 ANGPTL4 antibody (CL488-67577, Clone: 1D2H6 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated ANGPTL4 Monoclonal antibody

ANGPTL4 Monoclonal Antibody for IF/ICC
Cat No. CL488-67577

产品说明书

CloneNo. 1D2H6

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

IF/ICC

别名

Angiopoietin-related protein 4, Angiopoietin-like protein 4, Angiopoietin related protein 4, Angiopoietin like protein 4, angiopoietin like 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 67577-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67577 targets ANGPTL4 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 ANGPTL4 fusion protein Ag30070 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 angiopoietin-like 4
别名 Angiopoietin-related protein 4, Angiopoietin-like protein 4, Angiopoietin related protein 4, Angiopoietin like protein 4, angiopoietin like 4
计算分子量 45 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC023647
基因名称 ANGPTL4
Gene ID (NCBI) 51129
RRIDAB_2934554
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BY76
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three isoforms, 45 kDa, 41 kDa and 27 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ANGPTL4 antibody CL488-67577Download protocol
Standard Protocols
Click here to view our Standard Protocols