ANK3 Polyclonal antibody
ANK3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
27980-1-AP targets ANK3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human |
| Immunogen | ANK3 fusion protein Ag27635 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ankyrin 3, node of Ranvier (ankyrin G) |
| Synonyms | ANK 3, ANK3, Ankyrin 3, ANKYRIN G, FLJ45464 |
| Calculated Molecular Weight | 480 kDa |
| Observed Molecular Weight | 160 kDa, 204 kDa |
| GenBank Accession Number | NM_001149 |
| Gene Symbol | ANK3 |
| Gene ID (NCBI) | 288 |
| RRID | AB_2881028 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12955 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANK3 is associated with neurodevelopment and neuronal function. It has been reported that ANK3 plays a key role in bipolar disorder. ANK3 is expressed in brain at a high level. There are several isoforms of ANK3 associated with different tissue expression and function. The immune region we selected can recognize 480 kDa, 204 kDa, 202 kDa and 111 kDa protein and 27980-1-AP detects 204 kDa isoform in SDS-PAGE. (PMID: 27217151, 28687526, 30297702, 28109561, 30046097)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ANK3 antibody 27980-1-AP | Download protocol |
| IHC protocol for ANK3 antibody 27980-1-AP | Download protocol |
| IF protocol for ANK3 antibody 27980-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
