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验证数据展示

  • IF Staining of PC-3 using CL488-27980

    IF Staining of PC-3 using CL488-27980

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using CoraLite® Plus 488 ANK3 antibody (CL488-27980) at dilution of 1:200.

  • FC experiment of PC-3 using CL488-27980

    FC experiment of PC-3 using CL488-27980

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ANK3 (CL488-27980) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated ANK3 Polyclonal antibody

ANK3 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-27980

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

ANKYRING, Ankyrin3, ANKYRIN G, Ankyrin 3, ANK 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 27980-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27980 targets ANK3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 ANK3 fusion protein Ag27635 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin 3, node of Ranvier (ankyrin G)
别名 ANKYRING, Ankyrin3, ANKYRIN G, Ankyrin 3, ANK 3
计算分子量 480 kDa
观测分子量160 kDa, 204 kDa
GenBank蛋白编号NM_001149
基因名称 ANK3
Gene ID (NCBI) 288
RRIDAB_3084112
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12955
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANK3 is associated with neurodevelopment and neuronal function. It has been reported that ANK3 plays a key role in bipolar disorder. ANK3 is expressed in brain at a high level. There are several isoforms of ANK3 associated with different tissue expression and function. The immune region we selected can recognize 480 kDa, 204 kDa, 202 kDa and 111 kDa protein and 27980-1-AP detects 204 kDa isoform in SDS-PAGE. (PMID: 27217151, 28687526, 30297702, 28109561, 30046097)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ANK3 antibody CL488-27980Download protocol
FC protocol for CL Plus 488 ANK3 antibody CL488-27980Download protocol
Standard Protocols
Click here to view our Standard Protocols