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CoraLite® Plus 488-conjugated ANK3 Polyclonal antibody

ANK3 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-27980

Print datasheet

Synonyms

ANKYRING, Ankyrin3, ANKYRIN G, Ankyrin 3, ANK 3

验证数据展示

  • IF Staining of PC-3 using CL488-27980

    IF Staining of PC-3 using CL488-27980

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using CoraLite® Plus 488 ANK3 antibody (CL488-27980) at dilution of 1:200.

  • FC experiment of PC-3 using CL488-27980

    FC experiment of PC-3 using CL488-27980

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ANK3 (CL488-27980) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 27980-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27980 targets ANK3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen ANK3 fusion protein Ag27635 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin 3, node of Ranvier (ankyrin G)
Synonyms ANKYRING, Ankyrin3, ANKYRIN G, Ankyrin 3, ANK 3
Calculated Molecular Weight 480 kDa
Observed Molecular Weight160 kDa, 204 kDa
GenBank Accession NumberNM_001149
Gene Symbol ANK3
Gene ID (NCBI) 288
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12955
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANK3 is associated with neurodevelopment and neuronal function. It has been reported that ANK3 plays a key role in bipolar disorder. ANK3 is expressed in brain at a high level. There are several isoforms of ANK3 associated with different tissue expression and function. The immune region we selected can recognize 480 kDa, 204 kDa, 202 kDa and 111 kDa protein and 27980-1-AP detects 204 kDa isoform in SDS-PAGE. (PMID: 27217151, 28687526, 30297702, 28109561, 30046097)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ANK3 antibody CL488-27980Download protocol
FC protocol for CL Plus 488 ANK3 antibody CL488-27980Download protocol
Standard Protocols
Click here to view our Standard Protocols