验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rabbit heart tissue, pig heart tissue |
| Positive IF/ICC detected in | A431 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 555, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 2 publications below |
产品信息
67775-1-Ig targets ANKRD1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse |
| Immunogen | ANKRD1 fusion protein Ag29974 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ankyrin repeat domain 1 (cardiac muscle) |
| Synonyms | CVARP, CARP, C193, C 193, Ankyrin repeat domain-containing protein 1 |
| Calculated Molecular Weight | 319 aa, 36 kDa |
| GenBank Accession Number | BC018667 |
| Gene Symbol | ANKRD1 |
| Gene ID (NCBI) | 27063 |
| RRID | AB_2918540 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15327 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ANKRD1 antibody 67775-1-Ig | Download protocol |
| IF protocol for ANKRD1 antibody 67775-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
