CoraLite® Plus 647-conjugated ANKRD1 Monoclonal antibody
ANKRD1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat, rabbit, pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1F11F1
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
67775-1-Ig
For other applications, we recommend the unconjugated version of this antibody, 67775-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-67775 targets ANKRD1 in IF/ICC applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, mouse, rat, rabbit, pig |
| Immunogen | ANKRD1 fusion protein Ag29974 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ankyrin repeat domain 1 (cardiac muscle) |
| Synonyms | ALRP, ANKRD1, bA320F15.2, C 193, C193, Cardiac ankyrin repeat protein, CARP, CVARP, HA1A2, MCARP |
| Calculated Molecular Weight | 319 aa, 36 kDa |
| GenBank Accession Number | BC018667 |
| Gene Symbol | ANKRD1 |
| Gene ID (NCBI) | 27063 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15327 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 ANKRD1 antibody CL647-67775 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |