验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
67775-1-Ig
For other applications, we recommend the unconjugated version of this antibody, 67775-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-67775 targets ANKRD1 in IF/ICC applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, mouse, rat, rabbit, pig |
| 免疫原 | ANKRD1 fusion protein Ag29974 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ankyrin repeat domain 1 (cardiac muscle) |
| 别名 | ALRP, ANKRD1, bA320F15.2, C 193, C193, Cardiac ankyrin repeat protein, CARP, CVARP, HA1A2, MCARP |
| 计算分子量 | 319 aa, 36 kDa |
| GenBank蛋白编号 | BC018667 |
| 基因名称 | ANKRD1 |
| Gene ID (NCBI) | 27063 |
| RRID | AB_3084827 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15327 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 ANKRD1 antibody CL647-67775 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |