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Conjugate
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ANKRD54 Monoclonal antibody, PBS Only

ANKRD54 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E9A9

Cat no : 68388-1-PBS

Print datasheet

Synonyms

ANKRD54, ankyrin repeat domain 54

验证数据展示

  • WB analysis using 68388-1-Ig (same clone as 68388-1-PBS)

    WB analysis using 68388-1-Ig (same clone as 68388-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68388-1-Ig (ANKRD54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68388-1-PBS in a different storage buffer formulation.

  • WB analysis of U2OS using 68388-1-Ig (same clone as 68388-1-PBS)

    WB analysis of U2OS using 68388-1-Ig (same clone as 68388-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 68388-1-Ig (ANKRD54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68388-1-PBS in a different storage buffer formulation.

产品信息

68388-1-PBS targets ANKRD54 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ANKRD54 fusion protein Ag22772 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ankyrin repeat domain 54
Synonyms ANKRD54, ankyrin repeat domain 54
Calculated Molecular Weight 300 aa, 33 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberBC066909
Gene Symbol ANKRD54
Gene ID (NCBI) 129138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6NXT1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ankyrin repeat domain 54 (ANKRD54)/Liar is a nuclear-cytoplasmic shuttling adaptor that interacts with Lyn, Btk, Txk, and HCLS1. Activation of PKC kinases promoted nuclear export and phosphorylation of Ankrd54, and increased interaction and cytoplasmic co-localization with Lyn.