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验证数据展示

  • WB analysis using 67638-1-Ig

    WB analysis using 67638-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67638-1-Ig (ANO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 67638-1-Ig

    IHC staining of mouse eye using 67638-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67638-1-Ig (ANO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 67638-1-Ig

    IF Staining of mouse eye using 67638-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using ANO2 antibody (67638-1-Ig, Clone: 2F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

ANO2 Monoclonal antibody

ANO2 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67638-1-Ig

产品说明书

CloneNo. 2F12G1

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse

应用

WB, IHC, IF-P, ELISA

别名

ANO2, anoctamin 2, C12orf3, DKFZp434P102, TMEM16B, Transmembrane protein 16B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inY79 cells, NCCIT cells, U2OS cells, MDA-MB-231 cells, T-47D cells, HeLa cells
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67638-1-Ig targets ANO2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 anoctamin 2
别名 ANO2, anoctamin 2, C12orf3, DKFZp434P102, TMEM16B, Transmembrane protein 16B
计算分子量 114 kDa
观测分子量 114 kDa
GenBank蛋白编号NM_020373
基因名称 ANO2
Gene ID (NCBI) 57101
RRIDAB_2882839
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NQ90
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.

实验方案

Product Specific Protocols
WB protocol for ANO2 antibody 67638-1-IgDownload protocol
IHC protocol for ANO2 antibody 67638-1-IgDownload protocol
IF protocol for ANO2 antibody 67638-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols