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验证数据展示

  • WB analysis using 67638-1-Ig (same clone as 67638-1-PBS)

    WB analysis using 67638-1-Ig (same clone as 67638-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67638-1-Ig (ANO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 67638-1-Ig (same clone as 67638-1-PBS)

    IHC staining of mouse eye using 67638-1-Ig (same clone as 67638-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67638-1-Ig (ANO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse eye using 67638-1-Ig (same clone as 67638-1-PBS)

    IF Staining of mouse eye using 67638-1-Ig (same clone as 67638-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using ANO2 antibody (67638-1-Ig, Clone: 2F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67638-1-PBS in a different storage buffer formulation.

ANO2 Monoclonal antibody, PBS Only

ANO2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67638-1-PBS

产品说明书

CloneNo. 2F12G1

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

ANO2, anoctamin 2, C12orf3, DKFZp434P102, TMEM16B, Transmembrane protein 16B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67638-1-PBS targets ANO2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 Human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 anoctamin 2
别名 ANO2, anoctamin 2, C12orf3, DKFZp434P102, TMEM16B, Transmembrane protein 16B
计算分子量 114 kDa
观测分子量 114 kDa
GenBank蛋白编号NM_020373
基因名称 ANO2
Gene ID (NCBI) 57101
RRIDAB_2882839
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NQ90
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.