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ANP32A Monoclonal antibody, PBS Only

ANP32A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D8C5

Cat no : 67687-1-PBS

Print datasheet

Synonyms

Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1, Acidic leucine-rich nuclear phosphoprotein 32 family member A

验证数据展示

  • WB analysis using 67687-1-Ig (same clone as 67687-1-PBS)

    WB analysis using 67687-1-Ig (same clone as 67687-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67687-1-Ig (ANP32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67687-1-Ig (same clone as 67687-1-PBS)

    IHC staining of mouse brain using 67687-1-Ig (same clone as 67687-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANP32A antibody included
  • IHC staining of mouse brain using 67687-1-Ig (same clone as 67687-1-PBS)

    IHC staining of mouse brain using 67687-1-Ig (same clone as 67687-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANP32A antibody included
  • IF Staining of A431 using 67687-1-Ig (same clone as 67687-1-PBS)

    IF Staining of A431 using 67687-1-Ig (same clone as 67687-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32A antibody (67687-1-Ig, Clone: 2D8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 67687-1-Ig (same clone as 67687-1-PBS)

    IF Staining of A431 using 67687-1-Ig (same clone as 67687-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32A antibody (67687-1-Ig, Clone: 2D8C5 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ANP32A Ready-To-Use IHC Kit. ANP32A primary antibody included.

产品信息

67687-1-PBS targets ANP32A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ANP32A fusion protein Ag8546 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
Synonyms Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1, Acidic leucine-rich nuclear phosphoprotein 32 family member A
Calculated Molecular Weight 249 aa, 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC007200
Gene Symbol ANP32A
Gene ID (NCBI) 8125
RRIDAB_2882880
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP39687
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.