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验证数据展示

  • WB analysis using 67687-1-Ig

    WB analysis using 67687-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67687-1-Ig (ANP32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67687-1-Ig

    IHC staining of mouse brain using 67687-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANP32A antibody included
  • IHC staining of mouse brain using 67687-1-Ig

    IHC staining of mouse brain using 67687-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANP32A antibody included
  • IF Staining of A431 using 67687-1-Ig

    IF Staining of A431 using 67687-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32A antibody (67687-1-Ig, Clone: 2D8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of A431 using 67687-1-Ig

    IF Staining of A431 using 67687-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32A antibody (67687-1-Ig, Clone: 2D8C5 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

ANP32A Monoclonal antibody

ANP32A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67687-1-Ig

Print datasheet

CloneNo. 2D8C5
Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1, Acidic leucine-rich nuclear phosphoprotein 32 family member A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HSC-T6 cells, HuH-7 cells, HeLa cells, Raji cells, Jurkat cells, Sp2/0 cells
Positive IHC detected inmouse brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy ANP32A Ready-To-Use IHC Kit. ANP32A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67687-1-Ig targets ANP32A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Immunogen ANP32A fusion protein Ag8546 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
Synonyms Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1, Acidic leucine-rich nuclear phosphoprotein 32 family member A
Calculated Molecular Weight 249 aa, 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC007200
Gene Symbol ANP32A
Gene ID (NCBI) 8125
RRIDAB_2882880
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP39687
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.

实验方案

Product Specific Protocols
WB protocol for ANP32A antibody 67687-1-IgDownload protocol
IHC protocol for ANP32A antibody 67687-1-IgDownload protocol
IF protocol for ANP32A antibody 67687-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Sci Adv

The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals

Authors - Liuke Sun
View Article