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验证数据展示

  • WB analysis using CL594-67687

    WB analysis using CL594-67687

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-67687 (ANP32A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using CL594-67687

    IF Staining of A431 using CL594-67687

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 ANP32A antibody (CL594-67687, Clone: 2D8C5 ) at dilution of 1:200, CL488-Phalloidin (green).

CoraLite®594-conjugated ANP32A Monoclonal antibody

ANP32A Monoclonal Antibody for WB, IF/ICC
Cat No. CL594-67687

产品说明书

CloneNo. 2D8C5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IF/ICC

别名

Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1, Acidic leucine-rich nuclear phosphoprotein 32 family member A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, Raji cells, HeLa cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy ANP32A Ready-To-Use IHC Kit. ANP32A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67687-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67687 targets ANP32A in WB, IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 ANP32A fusion protein Ag8546 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
别名 Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1, Acidic leucine-rich nuclear phosphoprotein 32 family member A
计算分子量 249 aa, 29 kDa
观测分子量29 kDa
GenBank蛋白编号BC007200
基因名称 ANP32A
Gene ID (NCBI) 8125
RRIDAB_2920172
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP39687
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.

实验方案

Product Specific Protocols
WB protocol for CL594 ANP32A antibody CL594-67687Download protocol
IF protocol for CL594 ANP32A antibody CL594-67687Download protocol
Standard Protocols
Click here to view our Standard Protocols