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验证数据展示

  • WB analysis using CL488-67687

    WB analysis using CL488-67687

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67687 (ANP32A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using CL488-67687

    IF Staining of A431 using CL488-67687

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 ANP32A antibody (CL488-67687, Clone: 2D8C5 ) at dilution of 1:600, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated ANP32A Monoclonal antibody

ANP32A Monoclonal Antibody for WB, IF/ICC
Cat No. CL488-67687

Print datasheet

CloneNo. 2D8C5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC

Synonyms

Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1, Acidic leucine-rich nuclear phosphoprotein 32 family member A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, Raji cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy ANP32A Ready-To-Use IHC Kit. ANP32A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67687-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67687 targets ANP32A in WB, IF/ICC applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC Application Description
Tested Reactivity human
Immunogen ANP32A fusion protein Ag8546 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
Synonyms Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1, Acidic leucine-rich nuclear phosphoprotein 32 family member A
Calculated Molecular Weight 249 aa, 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC007200
Gene Symbol ANP32A
Gene ID (NCBI) 8125
RRIDAB_2919521
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP39687
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 ANP32A antibody CL488-67687Download protocol
IF protocol for CL Plus 488 ANP32A antibody CL488-67687Download protocol
Standard Protocols
Click here to view our Standard Protocols