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验证数据展示

  • IF Staining of A431 using 66160-1-Ig (same clone as 66160-1-PBS)

    IF Staining of A431 using 66160-1-Ig (same clone as 66160-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32B antibody (66160-1-Ig, Clone: 5A1F12 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66160-1-PBS in a different storage buffer formulation.

  • WB analysis using 66160-1-Ig (same clone as 66160-1-PBS)

    WB analysis using 66160-1-Ig (same clone as 66160-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66160-1-Ig (ANP32B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66160-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66160-1-Ig (same clone as 66160-1-PBS)

    WB analysis of HEK-293 using 66160-1-Ig (same clone as 66160-1-PBS)

    WB result of ANP32B antibody (66160-1-Ig, 1:5000) with si-control and si-ANP32B transfected HEK293 cells. This data was developed using the same antibody clone with 66160-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50363-1

    Cytometric bead array standard curve of MP50363-1

    Cytometric bead array standard curve of MP50363-1, ANP32B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66160-1-PBS. Detection antibody: 66160-2-PBS. Standard:Ag1274. Range: 1.563-200 ng/mL.

  • Cytometric bead array standard curve of MP50363-2

    Cytometric bead array standard curve of MP50363-2

    Cytometric bead array standard curve of MP50363-2, ANP32B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66160-1-PBS. Detection antibody: 66160-3-PBS. Standard:Ag1274. Range: 1.563-200 ng/mL.

  • Featured Product
  • KD/KO验证

ANP32B Monoclonal antibody, PBS Only (Capture)

ANP32B Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66160-1-PBS

产品说明书

CloneNo. 5A1F12

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Silver-stainable protein SSP29, Putative HLA-DR-associated protein I-2, PHAPI2, APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ANP32B Ready-To-Use IHC Kit. ANP32B primary antibody included.

产品信息

66160-1-PBS targets ANP32B as part of a matched antibody pair:

MP50363-1: 66160-1-PBS capture and 66160-2-PBS detection (validated in Cytometric bead array)

MP50363-2: 66160-1-PBS capture and 66160-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ANP32B fusion protein Ag1274 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
别名 Silver-stainable protein SSP29, Putative HLA-DR-associated protein I-2, PHAPI2, APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B
计算分子量 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC013003
基因名称 ANP32B
Gene ID (NCBI) 10541
RRIDAB_2881556
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92688
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).