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  • Featured Product
  • KD/KO Validated

ANP32B Monoclonal antibody, PBS Only

ANP32B Monoclonal Antibody for WB,Indirect ELISA,IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

5A1F12

Cat No : 66160-1-PBS

Print datasheet

Synonyms

ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29

验证数据展示

  • WB analysis using 66160-1-Ig (same clone as 66160-1-PBS)

    WB analysis using 66160-1-Ig (same clone as 66160-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66160-1-Ig (ANP32B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66160-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66160-1-Ig (same clone as 66160-1-PBS)

    WB analysis of HEK-293 using 66160-1-Ig (same clone as 66160-1-PBS)

    WB result of ANP32B antibody (66160-1-Ig, 1:5000) with si-control and si-ANP32B transfected HEK293 cells. This data was developed using the same antibody clone with 66160-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66160-1-Ig (same clone as 66160-1-PBS)

    IF Staining of HepG2 using 66160-1-Ig (same clone as 66160-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66160-1-Ig (ANP32B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66160-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ANP32B Ready-To-Use IHC Kit. ANP32B primary antibody included.

产品信息

66160-1-PBS targets ANP32B in WB, IF, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity human, mouse, rat
Immunogen ANP32B fusion protein Ag1274 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
Synonyms ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC013003
Gene Symbol ANP32B
Gene ID (NCBI) 10541
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92688
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).