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验证数据展示

  • IF Staining of A431 using 66160-1-Ig (same clone as 66160-1-PBS)

    IF Staining of A431 using 66160-1-Ig (same clone as 66160-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32B antibody (66160-1-Ig, Clone: 5A1F12 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66160-1-PBS in a different storage buffer formulation.

  • WB analysis using 66160-1-Ig (same clone as 66160-1-PBS)

    WB analysis using 66160-1-Ig (same clone as 66160-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66160-1-Ig (ANP32B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66160-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66160-1-Ig (same clone as 66160-1-PBS)

    WB analysis of HEK-293 using 66160-1-Ig (same clone as 66160-1-PBS)

    WB result of ANP32B antibody (66160-1-Ig, 1:5000) with si-control and si-ANP32B transfected HEK293 cells. This data was developed using the same antibody clone with 66160-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50363-1

    Cytometric bead array standard curve of MP50363-1

    Cytometric bead array standard curve of MP50363-1, ANP32B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66160-1-PBS. Detection antibody: 66160-2-PBS. Standard:Ag1274. Range: 1.563-200 ng/mL.

  • Cytometric bead array standard curve of MP50363-2

    Cytometric bead array standard curve of MP50363-2

    Cytometric bead array standard curve of MP50363-2, ANP32B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66160-1-PBS. Detection antibody: 66160-3-PBS. Standard:Ag1274. Range: 1.563-200 ng/mL.

  • Featured Product
  • KD/KO Validated

ANP32B Monoclonal antibody, PBS Only (Capture)

ANP32B Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66160-1-PBS

Print datasheet

CloneNo. 5A1F12

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

Silver-stainable protein SSP29, Putative HLA-DR-associated protein I-2, PHAPI2, APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ANP32B Ready-To-Use IHC Kit. ANP32B primary antibody included.

产品信息

66160-1-PBS targets ANP32B as part of a matched antibody pair:

MP50363-1: 66160-1-PBS capture and 66160-2-PBS detection (validated in Cytometric bead array)

MP50363-2: 66160-1-PBS capture and 66160-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ANP32B fusion protein Ag1274 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
Synonyms Silver-stainable protein SSP29, Putative HLA-DR-associated protein I-2, PHAPI2, APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC013003
Gene Symbol ANP32B
Gene ID (NCBI) 10541
RRIDAB_2881556
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92688
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).