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ANP32B Monoclonal antibody

ANP32B Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66160-1-Ig

产品说明书

CloneNo. 5A1F12

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

Silver-stainable protein SSP29, Putative HLA-DR-associated protein I-2, PHAPI2, APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inLNCaP cells, fetal human brain tissue, HEK-293 cells, mouse brain tissue, U2OS cells, A431 cells, HCT 116 cells, HeLa cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy ANP32B Ready-To-Use IHC Kit. ANP32B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:900-1:3600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66160-1-Ig targets ANP32B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 ANP32B fusion protein Ag1274 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
别名 Silver-stainable protein SSP29, Putative HLA-DR-associated protein I-2, PHAPI2, APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B
计算分子量 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC013003
基因名称 ANP32B
Gene ID (NCBI) 10541
RRIDAB_2881556
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92688
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).

实验方案

Product Specific Protocols
WB protocol for ANP32B antibody 66160-1-IgDownload protocol
IF protocol for ANP32B antibody 66160-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

Avian ANP32A incorporated in avian influenza A virions promotes interspecies transmission by priming early viral replication in mammals

Authors - Lei Na
humanWB

J Virol

ANP32 proteins are essential for influenza virus replication in human cells.

Authors - Ecco Staller
  • KO Validated
mouse,ratWB

Orphanet J Rare Dis

HDAC5 inhibition attenuates ventricular remodeling and cardiac dysfunction

Authors - Chenxi Zhu
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