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验证数据展示

  • IF Staining of HepG2 using CL488-66160

    IF Staining of HepG2 using CL488-66160

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ANP32B antibody (CL488-66160, Clone: 5A1F12 ) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ANP32B Monoclonal antibody

ANP32B Monoclonal Antibody for IF/ICC
Cat No. CL488-66160

Print datasheet

CloneNo. 5A1F12

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ANP32B Ready-To-Use IHC Kit. ANP32B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66160-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66160 targets ANP32B in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen ANP32B fusion protein Ag1274 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
Synonyms ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29
Calculated Molecular Weight 29 kDa
GenBank Accession NumberBC013003
Gene Symbol ANP32B
Gene ID (NCBI) 10541
RRIDAB_2919292
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92688
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ANP32B antibody CL488-66160Download protocol
Standard Protocols
Click here to view our Standard Protocols