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验证数据展示

  • IF Staining of HepG2 using CL488-66160

    IF Staining of HepG2 using CL488-66160

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ANP32B antibody (CL488-66160, Clone: 5A1F12 ) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated ANP32B Monoclonal antibody

ANP32B Monoclonal Antibody for IF/ICC
Cat No. CL488-66160

产品说明书

CloneNo. 5A1F12

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC

别名

ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ANP32B Ready-To-Use IHC Kit. ANP32B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66160-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66160 targets ANP32B in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 ANP32B fusion protein Ag1274 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
别名 ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29
计算分子量 29 kDa
GenBank蛋白编号BC013003
基因名称 ANP32B
Gene ID (NCBI) 10541
RRIDAB_2919292
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92688
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ANP32B antibody CL488-66160Download protocol
Standard Protocols
Click here to view our Standard Protocols