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Conjugate
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验证数据展示

  • WB analysis using 68726-1-Ig

    WB analysis using 68726-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68726-1-Ig (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68726-1-Ig

    IF Staining of HeLa using 68726-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AP1M1 antibody (68726-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

AP1M1 Monoclonal antibody

AP1M1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68726-1-Ig

产品说明书

CloneNo. 1E9A11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

AP-1 complex subunit mu-1, AP mu chain family member mu1A, AP 1 complex subunit mu 1, Adaptor-related protein complex 1 subunit mu-1, Adaptor protein complex AP-1 subunit mu-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-231 cells, A375 cells, NIH/3T3 cells, human placenta tissue, LNCaP cells, HEK-293 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68726-1-Ig targets AP1M1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 AP1M1 fusion protein Ag25303 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 adaptor-related protein complex 1, mu 1 subunit
别名 AP-1 complex subunit mu-1, AP mu chain family member mu1A, AP 1 complex subunit mu 1, Adaptor-related protein complex 1 subunit mu-1, Adaptor protein complex AP-1 subunit mu-1
计算分子量 423 aa, 49 kDa
观测分子量 47-49 kDa
GenBank蛋白编号BC017469
基因名称 AP1M1
Gene ID (NCBI) 8907
RRIDAB_3670418
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BXS5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

实验方案

Product Specific Protocols
WB protocol for AP1M1 antibody 68726-1-IgDownload protocol
IF protocol for AP1M1 antibody 68726-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols