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AP1M2 Polyclonal antibody

AP1M2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10618-1-AP

Print datasheet

Synonyms

AP1 mu2, AP-1 complex subunit mu-2, AP mu chain family member mu1B, AP 1 complex subunit mu 2, Adaptor-related protein complex 1 subunit mu-2

验证数据展示

  • WB analysis of Hacat cells using 10618-1-AP

    WB analysis of Hacat cells using 10618-1-AP

    WB result of AP1M2 antibody (10618-1-AP;1:500) with siRNA cell lysate.

  • WB analysis of mouse colon using 10618-1-AP

    WB analysis of mouse colon using 10618-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 10618-1-AP (AP1M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10618-1-AP

    IHC staining of human pancreas cancer using 10618-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 10618-1-AP

    IHC staining of human pancreas cancer using 10618-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse colon tissue, Hacat cells
Positive IHC detected inhuman pancreas cancer tissue, human kidney tissue, human bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10618-1-AP targets AP1M2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AP1M2 fusion protein Ag0961 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adaptor-related protein complex 1, mu 2 subunit
Synonyms AP1 mu2, AP-1 complex subunit mu-2, AP mu chain family member mu1B, AP 1 complex subunit mu 2, Adaptor-related protein complex 1 subunit mu-2
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC005021
Gene Symbol AP1M2
Gene ID (NCBI) 10053
RRIDAB_2242895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6Q5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AP1M2 is a subunit of the adaptor complex AP-1. It belongs to the adaptor complexes medium subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

实验方案

Product Specific Protocols
WB protocol for AP1M2 antibody 10618-1-APDownload protocol
IHC protocol for AP1M2 antibody 10618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols