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验证数据展示

  • WB analysis using 29887-1-AP

    WB analysis using 29887-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29887-1-AP (AP2A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 29887-1-AP

    IHC staining of human ovary tumor using 29887-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29887-1-AP (AP2A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AP2A1 antibody included
  • IHC staining of human ovary tumor using 29887-1-AP

    IHC staining of human ovary tumor using 29887-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29887-1-AP (AP2A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AP2A1 antibody included
  • IF Staining of HeLa using 29887-1-AP

    IF Staining of HeLa using 29887-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AP2A1 antibody (29887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of SH-SY5Y using 29887-1-AP

    IF Staining of SH-SY5Y using 29887-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using AP2A1 antibody (29887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

AP2A1 Polyclonal antibody

AP2A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29887-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

ADTAA, Alpha adaptin A, Alpha1 adaptin, AP 2 complex subunit alpha 1, AP2 ALPHA, AP2A1, CLAPA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, SH-SY5Y cells, mouse heart tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy AP2A1 Ready-To-Use IHC Kit. AP2A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29887-1-AP targets AP2A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse
免疫原 AP2A1 fusion protein Ag31809 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 2, alpha 1 subunit
别名 ADTAA, Alpha adaptin A, Alpha1 adaptin, AP 2 complex subunit alpha 1, AP2 ALPHA, AP2A1, CLAPA1
计算分子量108KD
观测分子量100-108 kDa
GenBank蛋白编号NM_014203
基因名称 AP2A1
Gene ID (NCBI) 160
RRIDAB_2918351
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95782
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.

实验方案

Product Specific Protocols
WB protocol for AP2A1 antibody 29887-1-APDownload protocol
IHC protocol for AP2A1 antibody 29887-1-APDownload protocol
IF protocol for AP2A1 antibody 29887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

J Biol Chem

The GPCR adaptor protein Norbin controls the trafficking of C5aR1 and CXCR4 in mouse neutrophils

Authors - Stephen A Chetwynd
View Article