AP2A1 Polyclonal antibody
AP2A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, SH-SY5Y cells, mouse heart tissue |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | SH-SY5Y cells, HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29887-1-AP targets AP2A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | Human, mouse |
Immunogen | AP2A1 fusion protein Ag31809 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | adaptor-related protein complex 2, alpha 1 subunit |
Synonyms | ADTAA, Alpha adaptin A, Alpha1 adaptin, AP 2 complex subunit alpha 1, AP2 ALPHA, AP2A1, CLAPA1 |
Calculated Molecular Weight | 108KD |
Observed Molecular Weight | 100-108 kDa |
GenBank Accession Number | NM_014203 |
Gene Symbol | AP2A1 |
Gene ID (NCBI) | 160 |
RRID | AB_2918351 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O95782 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for AP2A1 antibody 29887-1-AP | Download protocol |
IHC protocol for AP2A1 antibody 29887-1-AP | Download protocol |
IF protocol for AP2A1 antibody 29887-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |