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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 30030-1-AP (same clone as 30030-1-PBS)

    WB analysis using 30030-1-AP (same clone as 30030-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30030-1-AP (AP2A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30030-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 30030-1-AP (same clone as 30030-1-PBS)

    IHC staining of human stomach cancer using 30030-1-AP (same clone as 30030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30030-1-AP (AP2A1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30030-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AP2A1 antibody included

AP2A1 Polyclonal antibody, PBS Only

AP2A1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30030-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy AP2A1 Ready-To-Use IHC Kit. AP2A1 primary antibody included.

产品信息

30030-1-PBS targets AP2A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AP2A1 fusion protein Ag32232 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 2, alpha 1 subunit
别名
计算分子量 977 aa, 108 kDa
观测分子量108 kDa
GenBank蛋白编号BC014214
基因名称 AP2A1
Gene ID (NCBI) 160
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95782
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.