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Conjugate
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AP3D1 Polyclonal antibody, PBS Only

AP3D1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16454-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 16454-1-AP (same clone as 16454-1-PBS)

    WB analysis using 16454-1-AP (same clone as 16454-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 16454-1-AP (same clone as 16454-1-PBS)

    IF Staining of MCF-7 using 16454-1-AP (same clone as 16454-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AP3D1 antibody (16454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

产品信息

16454-1-PBS targets AP3D1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AP3D1 fusion protein Ag9390 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adaptor-related protein complex 3, delta 1 subunit
Synonyms
Calculated Molecular Weight 1153 aa, 130 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC010065
Gene Symbol AP3D1
Gene ID (NCBI) 8943
RRIDAB_10596951
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14617
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3D1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.