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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis of COLO 320 using 29022-1-AP (same clone as 29022-1-PBS)

    WB analysis of COLO 320 using 29022-1-AP (same clone as 29022-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 29022-1-AP (APAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29022-1-PBS in a different storage buffer formulation.

APAF1 Polyclonal antibody, PBS Only

APAF1 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 29022-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

29022-1-PBS targets APAF1 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 APAF1 fusion protein Ag30606 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apoptotic peptidase activating factor 1
别名
计算分子量 1248 aa, 142 kDa
观测分子量130-140 kDa
GenBank蛋白编号BC136532
基因名称 APAF1
Gene ID (NCBI) 317
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14727
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.