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验证数据展示

  • WB analysis of human plasma using 16845-1-AP

    WB analysis of human plasma using 16845-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 16845-1-AP (Apolipoprotein A II/APOA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of human plasma using 16845-1-AP

    IP experiment of human plasma using 16845-1-AP

    IP result of anti-Apolipoprotein A II/APOA2 (IP:16845-1-AP, 4ug; Detection:16845-1-AP 1:500) with human plasma lysate 4000 ug.

  • IHC staining of human liver using 16845-1-AP

    IHC staining of human liver using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16845-1-AP (Apolipoprotein A II/APOA2 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with APOA2 antibody included
  • IHC staining of human liver using 16845-1-AP

    IHC staining of human liver using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with APOA2 antibody included
  • IHC staining of human colon using 16845-1-AP

    IHC staining of human colon using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16845-1-AP (Apolipoprotein A II/APOA2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOA2 antibody included
  • IHC staining of human colon using 16845-1-AP

    IHC staining of human colon using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16845-1-AP (Apolipoprotein A II/APOA2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOA2 antibody included
  • IF Staining of HepG2 using 16845-1-AP

    IF Staining of HepG2 using 16845-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein A II/APOA2 antibody (16845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Apolipoprotein A II/APOA2 Polyclonal antibody

Apolipoprotein A II/APOA2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16845-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA

别名

APOA2, apolipoprotein A II, ApoA-II, Apo-AII, ApoA II

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue, human colon tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOA2 Ready-To-Use IHC Kit. APOA2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

16845-1-AP targets Apolipoprotein A II/APOA2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原 Apolipoprotein A II/APOA2 fusion protein Ag9863 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apolipoprotein A-II
别名 APOA2, apolipoprotein A II, ApoA-II, Apo-AII, ApoA II
计算分子量 100 aa, 11 kDa
观测分子量 7-9 kDa (monomer), 15 kDa (dimer)
GenBank蛋白编号BC005282
基因名称 APOA2
Gene ID (NCBI) 336
RRIDAB_2878324
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02652
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)

实验方案

Product Specific Protocols
WB protocol for Apolipoprotein A II/APOA2 antibody 16845-1-APDownload protocol
IHC protocol for Apolipoprotein A II/APOA2 antibody 16845-1-APDownload protocol
IF protocol for Apolipoprotein A II/APOA2 antibody 16845-1-APDownload protocol
IP protocol for Apolipoprotein A II/APOA2 antibody 16845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Authors - Yanying Wang
View Article
humanWB

Exp Cell Res

Extracellular vesicle-mediated transfer of the lncRNA-TC0101441 promotes endometriosis migration/invasion.

Authors - Jun-Jun Qiu
View Article
human,mouseWB

Int J Mol Sci

Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes

Authors - Zhonghao Li
View Article