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验证数据展示

  • WB analysis of human plasma using 16845-1-AP

    WB analysis of human plasma using 16845-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 16845-1-AP (Apolipoprotein A II/APOA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of human plasma using 16845-1-AP

    IP experiment of human plasma using 16845-1-AP

    IP result of anti-Apolipoprotein A II/APOA2 (IP:16845-1-AP, 4ug; Detection:16845-1-AP 1:500) with human plasma lysate 4000 ug.

  • IHC staining of human liver using 16845-1-AP

    IHC staining of human liver using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16845-1-AP (Apolipoprotein A II/APOA2 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with APOA2 antibody included
  • IHC staining of human liver using 16845-1-AP

    IHC staining of human liver using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with APOA2 antibody included
  • IHC staining of human colon using 16845-1-AP

    IHC staining of human colon using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16845-1-AP (Apolipoprotein A II/APOA2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOA2 antibody included
  • IHC staining of human colon using 16845-1-AP

    IHC staining of human colon using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16845-1-AP (Apolipoprotein A II/APOA2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOA2 antibody included
  • IF Staining of HepG2 using 16845-1-AP

    IF Staining of HepG2 using 16845-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein A II/APOA2 antibody (16845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Apolipoprotein A II/APOA2 Polyclonal antibody

Apolipoprotein A II/APOA2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16845-1-AP

Print datasheet

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

APOA2, apolipoprotein A II, ApoA-II, Apo-AII, ApoA II

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue, human colon tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOA2 Ready-To-Use IHC Kit. APOA2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

16845-1-AP targets Apolipoprotein A II/APOA2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen Apolipoprotein A II/APOA2 fusion protein Ag9863 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apolipoprotein A-II
Synonyms APOA2, apolipoprotein A II, ApoA-II, Apo-AII, ApoA II
Calculated Molecular Weight 100 aa, 11 kDa
Observed Molecular Weight 7-9 kDa (monomer), 15 kDa (dimer)
GenBank Accession NumberBC005282
Gene Symbol APOA2
Gene ID (NCBI) 336
RRIDAB_2878324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02652
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)

实验方案

Product Specific Protocols
WB protocol for Apolipoprotein A II/APOA2 antibody 16845-1-APDownload protocol
IHC protocol for Apolipoprotein A II/APOA2 antibody 16845-1-APDownload protocol
IF protocol for Apolipoprotein A II/APOA2 antibody 16845-1-APDownload protocol
IP protocol for Apolipoprotein A II/APOA2 antibody 16845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Authors - Yanying Wang
View Article
humanWB

Exp Cell Res

Extracellular vesicle-mediated transfer of the lncRNA-TC0101441 promotes endometriosis migration/invasion.

Authors - Jun-Jun Qiu
View Article
human,mouseWB

Int J Mol Sci

Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes

Authors - Zhonghao Li
View Article