• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HepG2 using CL488-16845

    IF Staining of HepG2 using CL488-16845

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Apolipoprotein A II antibody (CL488-16845) at dilution of 1:200.

CoraLite® Plus 488-conjugated Apolipoprotein A II/APOA2 Polyclonal antibody

Apolipoprotein A II/APOA2 Polyclonal Antibody for IF/ICC
Cat No. CL488-16845

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

APOA2, apolipoprotein A II, ApoA-II, Apo-AII, ApoA II

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOA2 Ready-To-Use IHC Kit. APOA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16845-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16845 targets Apolipoprotein A II/APOA2 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Apolipoprotein A II/APOA2 fusion protein Ag9863 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apolipoprotein A-II
Synonyms APOA2, apolipoprotein A II, ApoA-II, Apo-AII, ApoA II
Calculated Molecular Weight 100 aa, 11 kDa
Observed Molecular Weight 7-9 kDa (monomer), 15 kDa (dimer)
GenBank Accession NumberBC005282
Gene Symbol APOA2
Gene ID (NCBI) 336
RRIDAB_3672662
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02652
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Apolipoprotein A II/APOA2 antibody CL488-16845Download protocol
Standard Protocols
Click here to view our Standard Protocols