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Apolipoprotein A II Monoclonal antibody

Apolipoprotein A II Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G1A2

Cat no : 66773-1-Ig

Print datasheet

Synonyms

Apo AII, ApoA II, APOA2, apolipoprotein A II, Apolipoprotein A2

验证数据展示

  • WB analysis of human plasma using 66773-1-Ig

    WB analysis of human plasma using 66773-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66773-1-Ig (APOA2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66773-1-Ig

    IHC staining of human liver cancer using 66773-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66773-1-Ig (Apolipoprotein A II antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOA2 antibody included
  • IHC staining of human liver cancer using 66773-1-Ig

    IHC staining of human liver cancer using 66773-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66773-1-Ig (Apolipoprotein A II antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOA2 antibody included

经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy APOA2 Ready-To-Use IHC Kit. APOA2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66773-1-Ig targets Apolipoprotein A II in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen Apolipoprotein A II fusion protein Ag9863 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name apolipoprotein A-II
Synonyms Apo AII, ApoA II, APOA2, apolipoprotein A II, Apolipoprotein A2
Calculated Molecular Weight 100 aa, 11 kDa
Observed Molecular Weight 8-11 kDa
GenBank Accession NumberBC005282
Gene Symbol APOA2
Gene ID (NCBI) 336
RRIDAB_2882119
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)

实验方案

Product Specific Protocols
WB protocol for Apolipoprotein A II antibody 66773-1-IgDownload protocol
IHC protocol for Apolipoprotein A II antibody 66773-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols