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验证数据展示

  • FC experiment of HepG2 using CL594-66773

    FC experiment of HepG2 using CL594-66773

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Apolipoprotein A II/APOA2 (CL594-66773, Clone:1G1A2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated Apolipoprotein A II/APOA2 Monoclonal antibody

Apolipoprotein A II/APOA2 Monoclonal Antibody for FC (Intra)
Cat No. CL594-66773

产品说明书

CloneNo. 1G1A2

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

FC (Intra)

别名

APOA2, apolipoprotein A II, ApoA-II, Apo-AII, ApoA II

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOA2 Ready-To-Use IHC Kit. APOA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66773-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66773 targets Apolipoprotein A II/APOA2 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Apolipoprotein A II/APOA2 fusion protein Ag9863 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 apolipoprotein A-II
别名 APOA2, apolipoprotein A II, ApoA-II, Apo-AII, ApoA II
计算分子量 100 aa, 11 kDa
观测分子量 8-11 kDa
GenBank蛋白编号BC005282
基因名称 APOA2
Gene ID (NCBI) 336
RRIDAB_3084725
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02652
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)

实验方案

Product Specific Protocols
FC protocol for CL594 Apolipoprotein A II/APOA2 antibody CL594-66773Download protocol
Standard Protocols
Click here to view our Standard Protocols