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验证数据展示

  • WB analysis of human plasma using 66252-1-Ig (same clone as 66252-1-PBS)

    WB analysis of human plasma using 66252-1-Ig (same clone as 66252-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66252-1-Ig (APOA4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse small intestine using 66252-1-Ig (same clone as 66252-1-PBS)

    IHC staining of mouse small intestine using 66252-1-Ig (same clone as 66252-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse small intestine using 66252-1-Ig (same clone as 66252-1-PBS)

    IHC staining of mouse small intestine using 66252-1-Ig (same clone as 66252-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 66252-1-Ig (same clone as 66252-1-PBS)

    IF Staining of SH-SY5Y using 66252-1-Ig (same clone as 66252-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using APOA4 antibody (66252-1-Ig, Clone: 3A9A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66252-1-PBS in a different storage buffer formulation.

APOA4 Monoclonal antibody, PBS Only

APOA4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66252-1-PBS

产品说明书

CloneNo. 3A9A8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

APOA4, apolipoprotein A IV

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66252-1-PBS targets APOA4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 APOA4 fusion protein Ag12454 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 apolipoprotein A-IV
别名 APOA4, apolipoprotein A IV
计算分子量 396 aa, 45 kDa
观测分子量 45-46 kDa
GenBank蛋白编号BC074764
基因名称 APOA4
Gene ID (NCBI) 337
RRIDAB_2881640
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP06727
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote INS secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.