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APOA4 Monoclonal antibody

APOA4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3A9A8

Cat no : 66252-1-Ig

Print datasheet

Synonyms

APOA4, apolipoprotein A IV

验证数据展示

  • WB analysis of human plasma using 66252-1-Ig

    WB analysis of human plasma using 66252-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66252-1-Ig (APOA4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 66252-1-Ig

    IHC staining of mouse small intestine using 66252-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 66252-1-Ig

    IHC staining of mouse small intestine using 66252-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66252-1-Ig (APOA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 66252-1-Ig

    IF Staining of SH-SY5Y using 66252-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using APOA4 antibody (66252-1-Ig, Clone: 3A9A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected inhuman plasma tissue, L02 cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66252-1-Ig targets APOA4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen APOA4 fusion protein Ag12454 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name apolipoprotein A-IV
Synonyms APOA4, apolipoprotein A IV
Calculated Molecular Weight 396 aa, 45 kDa
Observed Molecular Weight 45-46 kDa
GenBank Accession NumberBC074764
Gene Symbol APOA4
Gene ID (NCBI) 337
RRIDAB_2881640
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06727
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote INS secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.

实验方案

Product Specific Protocols
WB protocol for APOA4 antibody 66252-1-IgDownload protocol
IHC protocol for APOA4 antibody 66252-1-IgDownload protocol
IF protocol for APOA4 antibody 66252-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols