• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Biotin-conjugated APOA4 Monoclonal antibody

APOA4 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IHC

Conjugate

Biotin

CloneNo.

3A9A8

Cat no : Biotin-66252

Print datasheet

Synonyms

APOA4, apolipoprotein A IV

验证数据展示

  • IHC staining of mouse small intestine using Biotin-66252

    IHC staining of mouse small intestine using Biotin-66252

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using Biotin-66252 (APOA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-66252 targets APOA4 in IHC applications and shows reactivity with human, mouse samples.

Tested Applications IHC Application Description
Tested Reactivity human, mouse
Immunogen APOA4 fusion protein Ag12454 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name apolipoprotein A-IV
Synonyms APOA4, apolipoprotein A IV
Calculated Molecular Weight 396 aa, 45 kDa
Observed Molecular Weight 45-46 kDa
GenBank Accession NumberBC074764
Gene Symbol APOA4
Gene ID (NCBI) 337
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06727
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote INS secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.

实验方案

Product Specific Protocols
IHC protocol for Biotin APOA4 antibody Biotin-66252Download protocol
Standard Protocols
Click here to view our Standard Protocols